Basic Laboratory Methods for Biotechnology

Basic Laboratory Methods for Biotechnology Author Lisa A. Seidman
ISBN-10 0321570146
Release 2009
Pages 789
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Presented from the perspective of the biotech industry, this laboratory handbook/textbook reference gives a systematic, understandable, and practical introduction to fundamental laboratory methods and provides a foundation upon which students can build a career in the lab. The authors balance background and theory with practical information, drawing material from many sources: analytical chemistry texts, molecular biology manuals, industry standards, government regulations, manufacturer and supplier information, and the useful laboratory “lore” that is part of the industry's oral tradition.The Modern Biotechnology Industry: A Broad Overview, The Business of Biotechnology: The Transformation of Knowledge into Products, Pharmaceutical/Biopharmaceutical Products, Introduction to Product Quality Systems, Biotechnology and the Regulation of Food and Medical Products, Documentation, the Foundation of Quality, Quality Systems in the Production Facility, Quality Systems in the Laboratory, Introduction to a Safe Workplace, Working Safely in the Laboratory: General Considerations and Physical Hazards, Working Safely with Chemicals, Working Safely with Biological Materials, Basic Math Techniques, Proportional Relationships, Relationships and Graphing, Descriptions of Data (Descriptive Statistics),Introduction to Quality Laboratory Measurements, Tests and Assays, Introduction to Instrumental Methods and Electricity,The Measurement of Weight, The Measurement of Volume, The Measurement of Temperature, The Measurement of pH, Selected Ions and Conductivity, Measurements Involving Light A. Basic Principles and Instrumentation, Introduction to Quality Laboratory Tests and Assays, Measurements Involving Light B. Applications and Methods, Preparation of Laboratory Solutions A: Concentration Expressions and Calculations, Preparation of Laboratory Solutions B. Basic Procedures and Practical Information, Solutions: Associated Procedures and Information, Laboratory Solutions to Support the Activity of Biological Macromolecules, Culture Media for Intact Cells, Introduction to Filtration, Introduction to Centrifugation, Introduction to Bioseparations, Computers: An Overview, Data Handling with Computers, Applications of the Internet to Biotechnology.Itended for those interested in learning the basics of laboratory methods for biotechnology



Basic Laboratory Methods for Biotechnology

Basic Laboratory Methods for Biotechnology Author Lisa A. Seidman
ISBN-10 0137955359
Release 2000-01
Pages 751
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A primary text for courses that focus on basic laboratory methods in biotechnology, and an ancillary text for any biology course that includes a laboratory component that want to focus on biotechnology. It provides an introduction to basic laboratory methods from the perspective of everyday work in the labs of biotechnology/bioscience industry.



Basic Laboratory Calculations for Biotechnology

Basic Laboratory Calculations for Biotechnology Author Lisa A. Seidman
ISBN-10 UCSD:31822034289918
Release 2008
Pages 482
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To succeed in the lab, it is crucial to be comfortable with the math calculations that are part of everyday work. This accessible introduction to common laboratory techniques focuses on the basics, helping even readers with good math skills to practice the most frequently encountered types of problems. Discusses very common laboratory problems, all applied to real situations. Explores multiple strategies for solving problems for a better understanding of the underlying math. Includes hundreds of practice problems, all with solutions and many with boxed, complete explanations; plus hundreds of “story problems” relating to real situations in the lab. MARKET: A useful review for biotechnology laboratory professionals.



Basic Methods for the Biochemical Lab

Basic Methods for the Biochemical Lab Author Martin Holtzhauer
ISBN-10 9783540327868
Release 2006-09-13
Pages 252
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This book presents proven lab procedures and practical hints for research in analytical and preparative biochemistry, and offers convenient key data in numerous tables. Coverage includes quantitative methods; electrophoresis; chromatographic protocols; immunochemical protocols; centrifugation; and radioactivity. In additional chapters, tables offer quick access to a broad array of useful information, including SI units conversion factors; detergent, protein and nucleotide data; and the basic principles of statistics and enzyme and receptor kinetics are reviewed. This first English-language edition of a successful German-language manual is a valuable resource for students and working professionals in biochemistry, biotechnology and biomedical laboratories.



Calculations for Molecular Biology and Biotechnology

Calculations for Molecular Biology and Biotechnology Author Frank H. Stephenson
ISBN-10 9780128025987
Release 2016-06-16
Pages 496
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Calculations in Molecular Biology and Biotechnology, Third Edition, helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables. In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work. Features comprehensive calculations in biotechnology and molecular biology experiments from start to finish Provides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Includes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the text Features new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteins Includes more sample problems in every chapter for readers to practice concepts



Basic Bioscience Laboratory Techniques

Basic Bioscience Laboratory Techniques Author Philip L.R. Bonner
ISBN-10 9781119956440
Release 2011-08-24
Pages 232
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This unique, practical, pocket-sized guide and reference provides every first year bioscience student with all they need to know to prepare reagents correctly and perform fundamental laboratory techniques. It also helps them to analyse their data and present their findings, in addition to directing the reader, via a comprehensive list of references, to relevant further reading All of the core bioscience laboratory techniques are covered including: basic calculations and the preparation of solutions; aseptic techniques; microscopy techniques; cell fractionation ; spectrophotometry; chromatography of small and large molecules: electrophoresis of proteins and nucleic acids and data analysis. In addition the book includes clear, relevant diagrams and worked examples of calculations. In short, this is a 'must-have' for all first year bioscience students struggling to get to grips with this vitally important element of their course.



Natural Products Isolation

Natural Products Isolation Author Satyajit D. Sarker
ISBN-10 9781592599554
Release 2005
Pages 515
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Natural Products Isolation: Second Edition presents a practical overview of just how natural products can be extracted, prepared, and isolated from the source material. Maintaining the main theme and philosophy of the first edition, this second edition incorporates all the new significant developments in this field of research. The chapters are divided into four distinct sections: introduction, extraction, chromatography, and special topics. This second edition provides substantial backround information for natural product researchers and will prove a useful reference guide to all of the available techniques.



Laboratory Manual for Biotechnology and Laboratory Science

Laboratory Manual for Biotechnology and Laboratory Science Author Lisa A. Seidman
ISBN-10 0321644026
Release 2010-10-27
Pages 433
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Laboratory Manual for Biotechnology provides the basic laboratory skills and knowledge to pursue a career in biotechnology. The manual, written by four biotechnology instructors with over 20 years of teaching experience, incorporates instruction, exercises, and laboratory activities that the authors have been using and perfecting for years. These exercises and activities serve to engage and help you understand the fundamentals of working in a biotechnology laboratory. Building skills through an organized and systematic presentation of materials, procedures, and tasks, the manual will help you explore overarching themes that relate to all biotechnology workplaces. The fundamentals in this manual are critical to the success of research scientists, scientists who develop ideas into practical products, laboratory analysts who analyze samples in forensic, clinical, quality control, environmental, and other testing laboratories.



Molecular Biology and Biotechnology

Molecular Biology and Biotechnology Author M. P. Bansal
ISBN-10 9788179933794
Release 2013-01-01
Pages 392
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Molecular Biology and Biotechnology: basic experimental protocols is a compilation of methods and techniques commonly used in biomedical and biotechnological studies. The book aims to provide ample support to both students and faculty while conducting practical lessons. Four sections are covered in this book—Genomics, Proteomics, Quantitative Biochemistry, and Bioinformatics. A concise introductory note accompanies each protocol/method described for better comprehension. Every topic discussed is supported by actual methods and their expected results, and is accompanied by relevant questions.



Molecular Biology Techniques

Molecular Biology Techniques Author Dr. Sue Carson
ISBN-10 9780123855442
Release 2012
Pages 200
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project? approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions



Environmental Microbiology

Environmental Microbiology Author J. F. T. Spencer
ISBN-10 9781592597659
Release 2004
Pages 423
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A collection of readily reproducible techniques for determining most of the important factors governing microorganisms and their habitats. Presented in step-by-step detail, these cutting-edge methods range from those for the study of marine organisms, to those for investigating microorganisms occurring in groundwater, to the biodiversity of yeasts found in remote environments. The novel protocols for studying fermented milks are significant for investigators concerned with milk as an item of food for infants, small children, and even adults. Additional methods for the recovery and determination of nucleic acids and other compounds affecting, and affected by, microorganisms are given for certain enzymes produced by plant pathogens and for obtaining microbial species tolerant of such inhibitors as heavy metals.



Molecular Biology Techniques

Molecular Biology Techniques Author Walt Ream
ISBN-10 9780080536828
Release 1998-11-17
Pages 234
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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter



Methods in Biotechnology

Methods in Biotechnology Author Seung-Beom Hong
ISBN-10 9781119156796
Release 2016-05-12
Pages 360
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As rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods; Methods in Biotechnology is an invaluable resource for those students and professionals. Methods in Biotechnology engages the reader by implementing an active learning approach, provided advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills. This text is broken into three sections based on level – Methods in Biotechnology, Advanced Methods in Biotechnology I, and Advanced Methods in Biotechnology II. Each section contains 14-22 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field.



Practical Biotechnology Methods Protocols

Practical Biotechnology Methods   Protocols Author S Janarthanam
ISBN-10 8173715823
Release 2007
Pages 136
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The Book Helps Undergraduate, Postgraduate And Research Students To Perform Basic Experiments In Biotechnology. The Laboratory Protocols Are Simple To Understand By Students From Different Scientific Backgrounds. Each Laboratory Exercise Contains An Introductory Unit, Protocol And Easy-To-Follow Instructions For Reagent Preparation. The Methods And Protocols Given Here Aim To Make Students Ready For Independent Research In Biotechnology Laboratories.



Basic Science Methods for Clinical Researchers

Basic Science Methods for Clinical Researchers Author Morteza Jalali
ISBN-10 9780128030783
Release 2017-03-31
Pages 382
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Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)



Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology Author Keith Wilson
ISBN-10 9780521516358
Release 2010-03-04
Pages 744
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Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.



Basic Techniques in Molecular Biology

Basic Techniques in Molecular Biology Author Stefan Surzycki
ISBN-10 9783642569685
Release 2012-12-06
Pages 434
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This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.